제왕절개수술시 무균적으로 얻은 탈락막조직세포를 E.coli의 내독소인 LPS로 자극하여 이 세포에서 분비되는 IL-1β와 PG를 측정하고 IL-1β에 의한 PG 생성여부를 측정하여 LPS 의하여 PG가 생성되는 과정에서 IL-1β의 역할을 밝히고 monoclonal mouse antihuman IL-1β와 TGF-β2에 의하여 PG생성의 억제가 이루어지는가를 실험하여 다음과 같은 결과를 얻었다. 1. 탈락막세포의 배양액에 LPS를 첨가하고 24시간후에 측정한 PGE2와 IL-1β는 각각 대조군에 비하여 통계적으로 유의하게 증가하였다. 2. 탈락막세포의 배양액에 IL-1β를 첨가하고 24시간 후에 측정한 PGE2는 대조군에 비하여 통계적으로 유의하게 증가하였다. 3. 탈락막세포의 배양액에 LPS 및 anti-IL-1β는 LPS를 첨가한 군에 비하여 통계적으로 유의하게 감소하였다. 4. 탈락막세포의 배양액에 LPS 및 TGF-β2를 첨가하고 24시간후에 측정한 PGE2치는 LPS를 첨가한 군에 비하여 통계적으로 유의하게 감소하였으나 IL-1β는 유의하게 증가하였다. 결론적으로 탈락막 조직세포내에서 LPS에 의하여 PG가 생성될 때 IL-1β가 부분적으로 관여함을 알 수 있었고, anti-IL-1β ALC TGF-β2는 PG 생성을 억제하는 것으로 보아 조기분만진통의 억제에 사용 가능할 것으로 생각된다.

The mechanisms responsible for the onset of preterm labor in women are poorly understood. It is widely accepted that increased biosynthesis of PGE2 by intrauterine tissues seems to be a key event in the initiation of preterm parturition. However, the mechanisms for the increased PG formation during parturition have not yet been explained. Growing evidednce suggesis an association between intraamniotic infection and preterm labor. It is suggested that bacterial products can signal the increased PG biosynthesis associated with parturition, and that decidua can induce the onset of preterm labor by using inflammatory mediators(for example, IL-1β) produced in response to bacterial invasion.The purposes of this study were to determine the effect of LPS on the production of IL-1β and PGE2 and to determine the inhibitory effect of anti-IL-1β and TGF-β2 on the production of IL-1β and PGE2 BY HUMAN DECIDUAL CELLS.The results were as follows : 1. The production of PGE2 AND IL-β by decidual cells after incubation with LPS for 24 hours culture significantly increased in comparison with controls, respectively.2. The production of PGE2 by decidual cells after incubation with il-1β for 24 hours culture significantly increased in comparision with controls.3. The production of PGE2 by decidual cells after incubation with LPS and anti-IL-1β for 24 hours culture significantly decreased in comparision with LPS treated groups, respectively.4. The production of PGE2 by decidual cells after incubation with LPS and TGF-β2 for 24 hours culture significantly decreased, but IL-1β production significantly increased in comparision with LPS treated groups.In conclusion, LPS may induce the formation PGE2 through IL-1β, and LPS induced preterm labor may be prevented by anti-IL-1β and TGF-β2.