Gardnerella vaginalis의 성장에 관한 일반적인 조건들과 임상검체에서 분리된 야생균주들의 생 물형, 보관조건에 따른 생존율을 시험한 결과 다음과 같은 결과를 얻었다. 1. 각 종 동물혈에 대한 G. vaginalis의 용혈능을 시험한 결과 인혈, 마혈, 양혈에 대하여 용혈 능력이 있었으며, 양혈에 대한 용혈능이 가장 높았다. 2. G. vaginalis의 증식에 적당한 고체배지는 Casmans agar에 5% Filedes enrichment와 5% horse serum을 넣은 배지였다. 3. Casmans agar에 X와 V인자만을 넣은 배지에서 균의 성장이 없었기 때문에 X와 V인자외 의 다른 성장인자를 필요로 한다. 4. 액체배지에서 가장 높은 성장율을 나타낸 배지는 Csamas agar에 용혈인혈을 10% 넣은 CTH10배지였다. 5. 각 액체배지에서 성장곡선을 그린 결과 지연기는 대략 6시간이었으며 정상기는 약 20시 간 정도였다. 6. 임상검체에서 분리된 야생균주의 생물형은 1, 2, 3, 4, 5, 7이었으며 6과 8은 형별되지 않았 다. 15균주중 가장 많은 생물형은 7이었다. 7. 균주의 단기보관시 가장 오래동안 생존하는 보관법은 혐기성 냉장 보관 상태로써 28일이 상 생존하였다.

We have studied general growth conditions of Gardnerella vaginalis, which is the predominant vaginal microorganism in women with bacterial vaginosis, and carried out biotyping of 15 wild strains isolated from bacterial vaginosis patients. Standard strain (ACTT 14018) of G. vaginalis had hemolytic activity to human blood, horse blood and sheep blood, but there were no hemolysis on geese, duck, beef and rabbit blood agar. We have confirmed that transparent solid media adequate for good growth of G. vaginalis was Casmans agar supplemented with 5% Fildes enrichment and 5% horse serum. There was no visible colonial growth on Casmans agar patched with X(Haemin) and V(nicotinamide adenine dinucleotide) factor strips. It means that G. vaginalis needs another unknown growth factor besides X and V factors G. vaginalis have exhibited best growth in Casmans broth supplemented with 10% freezing- thawing lysed human blood after 48 hours incubation, when growth was measured by optical density. In growth curve checked by the hour (Fig. 1), lag phase was about 6 hours after inoculation and growth was attained to stationary phase about 20 hours after inoculation. In biotyping, we have identified biotype 1,2,3,4,5,7 among eight kinds of biotypes, biotype 6, 8 were not typed, and type 7 was most common biotype(5/15 strains). Finally we have observed that this bacteriae had survived for 28 days more at 4。C anaerobic storage condition. It that this short period storage condition is more suitable for short survival of bacteriae than another three conditions (Table 5).