본 연구방법과 같이 일정한 검색(screening)단계를 거쳐 인간 신선정자보다 SPA결과의 분산 정도가 낮은 소 냉동 정자 표본만을 선택하여 이를 SPA에서 대조정자로 사용한다면 분석 자체의 실험 기법상의 오차로 인한 위음성 결과(false-negative result)를 확실히 배제할 수 있으므로, 임상적으로 남성의 가임능력을 평가하기 위해 시행한 SPA검사 결과의 판정에 보다 신중을 기할 수 있을 것으로 사료된다.

To determine the interassay variation in hamster ova penetration and to establish quality control measure for sperm penetration assay protocol using low temperature capacitation in TEST-yolk buffer, frozen sperm samples collected from 4 fertile bull control and fresh sperm samples collected from 2 healthy human donors were evaluated concurrently in the sperm penetration assay on 10 occasions and the assay results were analyzed. Hamster ova penetration of human sperm showed significantly greater variance and coefficient of variation than those of frozen bull sperm except those of bull-3. Interassay coefficient of variation of sperm penetration assay results of all human sperm was found to be 14.5% and those of all bull sperm was 13.8%. These results suggest that bull sperm can be used as a reliable alternative to human sperm as an internal control in the sperm penetration assay.