체내수정된 생쥐 배자의 체외성장 , 신생아 제대혈청 , 정도관리

To establish quality control system of fetal cord serum in human in vitro fertilization procedure, the possibility of using preimplantation mouse embryo system was investigated. Using ICR mice in vitro culture of preimplantation embryo from the two-cell stage to blastocyst, was carried out mixing fetal cord serum to Ham`s F-10. And the following results were obtained. 1. The vaginal mucus plug formations were observed 74.0%(148/200) of mice which were mated after superovulation. 2. Two-cell embryos retrieved following flushing of oviducts were 14.9 2.8 per mouse after superovulation. 3. In comparison with two media after 72 hours in vitro culture, cleavage rate of morula and blastocyst were 59.8% in Ham`s F-10 and 66.2% in m-KRB. m-KRB medium was superior to Ham`s F-10 to support mouse embryo in vitro. 4. Using the mouse embryo culture system, tests on th preparation of fetal cord serum resulted in 71.7% producing more than 60% morula or blastocyst stages after 72 hours in culture. It was concluded that mouse embryo system in vitro by using Ham`s F-10 mixed with fetal cord serum has a valuable place for the quality control of culture media in human in vitro fertilization program.