여지전기영동법 및 한천이중토역확산법을 이용하여 20예의 정상분만시의 양수단백분획 및 immunoglobulin의 성상을 관찰한 바 다음과 같은 결론을 얻었다. 1) 양수내의 총단백량은 263.8±16.5mg/dl이었으나 모체혈청과 태아혈청의 총단백량은 각각 7.3±0.2g/dl과 6.5±0.3g/dl로 양수단백보다 훨씬 높았다. 2) 양수단백의 분획에 의한 A/G비는 1.3이었으며, globulin 가운데 beta-globulin이 16.1± 0.3%로 가장 높았으며, 양수단백의 각분획의 백분비는 모체혈청단백의 분획과 유사하였다. 3) 양수 및 태아혈청에 대하여 anti-IgG 및 IgM에서는 침강반응선을 관찰할 수 있었으나 anti-IgA에서는 침강반응선을 관찰할 수 없었다. 따라서 양수단백내에는 IgG 및 IgM이 존재함을 알 수 있으며 IgA는 한천이중토역확산법으로 검출할 수 있는 충분한 양이 없음을 알 수 있다.

The origin and formation of amniotic fluid have been the subject of many scientific investifations. More than 20 different theories have been proposed in attempts to explain the formation of the amniotic fluid but only few have survived the test of time. Although the mechanical function and physiochemical view of the amniotic fluid has been known well, the immunological and biochemical function of amniotic fluid are obscure at present time. Electropheresis techniques and antigenic analysis have demonstrated the presence of serum proteins in umbilical cord, maternal serum and amniotic fluid during pregnancy and have stimulated investigations concerned with their origin and the factors which regulate their presence and concentration. The evidence to date, from both in vivo and in vitro studies suggest that the paper electropheric patterns of amniotic fluid proteins were simillar to that of maternal serum. Utilizing the techniques of paper electropheric and agar gel diffusion method, concentrations in amniotic fluid, meternal and cord serum have been analyzed in the term pregnant women. The results are summarized as follows : 1) The total amount of protein in the amniotic fluid is 263.8±16.5mg/dl compared with 1.3±0.2g/dl in the maternal serum and 6.5±0.3g/dl in the umbilical cord serum. 2) The fractions of amniotic fluid protein are similar to that of maternal serum by means of paper electropheresis and the fraction of albumin amniotic fluid is 57.0± 0.5mg/dl. 3) By the agar-gel difusion methos, immunoglobulin G and M are detected in both amniotic fluid and fetal cord blood but 1g A is not detected.