The present study was undertaken for the purpose of determining the pattern of urinary protein excretion in pre-eclampsia and eclampsia condition and for evaluating this technique as a mean for their differentiation. The serum and urine collection was done simultaneously from 13 pre-eclampsia and eclampsia patient before any medication. The electrophorectic separation of urinary and serum protein was carried out simultaneously by modified Wiere`s method. The results were as follows: 1) The fall in serum total protein, albumin, and rise in total globulin, especially α-globulins, were observed in toxemia cases in comparison with normal pregnancy at term. 2) The urine protein fractionations contained all the corresponding serum protein components and showed no evidence of special protein present or absence of certain fraction. 3) In the integral curve of urine protein fractionation, albumin and α-globulin peaks were higher than corresponding serum samples, and typical peak of β-globulin was prominent. 4) The more severe was proteinuria, the more promient was urinary α1-globulin. 5) Correlation between serum protein ＆ urinary excreted protein was statistically significant at the 5% level of significance in all fractions, and it is presumed that mechanism of proteinuria in toxemia is probably due to increased glomerular capillary permeability and decreased selective re-absorption from renal tubule by unknown toxemia factors.