1. 정상임부뇨에서는 histidine 뇨반응이 60% tryptophan 뇨반응이 78.5%의 적중율을 얻었고 임신 3개월이내에서는 histidine이 30%, tryptophan이 70%의 적중율로서 tryptophan이 histidine보다 임신과 더 밀접한 관계가 있음을 알았다. 2. 자간전증환자뇨에서는 histidine이 전예에서 음성반응을 보였다. 3. 병적임신뇨에서는 histidine 및 tryptophan이 전부 저율을 나타내었고 병적임신뇨에 대한 임신이상반응은 약 50%의 양성성적을 보았다. 4. 욕부뇨에 대한 histidine 및 tryptophan 뇨반응은 분만후 시일이 지날수록 양성율이 저하되어 적어도 분만후 1주일이내에는 거의 전예에서 음성화함을 볼 수 있었다. 5. 비임부뇨, 남성뇨에 대한 histidine 및 tryptophan 반응은 남녀간에 특별한 차이를 볼 수 없었다. 6. 이상의 성적으로 보아 histidine은 임신조기진단에는 이용가치가 없으며 오히려 임신시 물질대사의 변화를 관찰하는데 의의가 있고 tryptophan은 임신과 더 밀접한 관계가 있으나 임상적이용에는 앞으로의 연구가 필요하다고 생각한다.

A simplified method for the identification of urinary histidine (Kapeller-Adler reaction), tryptophan and the color reactions for the derivative of its metabolite indol (Ashitaka reaction) was tested. The test was performed using a uring specimen from 120 normal pregnant women, 52 non-pregnant women, 40 male adults, 8 girls and boys, 30 puerpera, and 39 pregnancy abnormalities such as preeclampsia, ectopic gestation, abortion and premature delivery. Results: 1) Of 120 normal pregnant urine tests, there were 72 positive responses of histidine and 94 positive responses of tryptophan. In the first trimester of pregnancy the accuracies of 30% and 70% were obtained for histidine and tryptophan respectively. The increased excretions of urinary histidine were not found before the third month of pregnancy and cannot, therefore, be used as an aid in the early diagnosis of pregnancy. It was also experimentally shown that tryptophan was more closely related to pregnancy. 2) It was interesting that all cases of preeclampsia showed negative responses for histidine but 50% positive responses for tryptophan. 3) The histidine and tryptophan tests in the urine of pregnancy abnormalities showed low positive responses (about 50%) and pregnancy abnormality reaction was positive in about 50 percent. 4) In the test of puerperal women urine, the response became gradually negative from the second postpartum day and at least almost completely negative within the seventh postpartum day. 5) There was no detectable difference in histidine and tryptophan reaction between non-pregnant women and men.