저자는 뇨중 E의 화학적정량법에 의하여 정상월경주기를 가진 부인 7예에서 월경주기를 월경기, 난포기, 배란기, 황체기의 4예로 나누어 분획측정하였고 월경주기이상환자 11예에서 분획측정한 결과 그 성적은 대략 다음과 같다. 1) 정상월경주기를 가진 부인의 뇨중 E는 월경기에 가장 적고 난포기에 증가하여 배란기에 Peak를 정하고 황체기에 감소한다. 뇨중 E의 총평균은 91γ/24hrs이다. 2) 총발성무월경환자 5예중 3예는 E치가 낮고 2예는 정상이며 뇨발성무월경환자의 2예는 모두 낮다. 3) 월경불순환자 4예에서는 전예 정상범위내에 있다. 4) Estradiol, Estrone, Estriol의 각분획은 정상월경주기를 가진 부인 및 월경주기이상환자에 있어서 Estradiol이 가장 적고 Estrone은 그보다 많으며 Estriol이 가장 많은 경향을 보인다.

The existing biological methods for the evalustion of estrogen have been complicated, inaccurate, furthermore, non-specific in procedures. The recent advances in the chromatography has brought the chemical methods for the evaluation of the chemical estrogen I.D. estrone, estradiol and estriol by fractional assesment. As a part of the research project in this deportment related to the various tests for the evaluation of the functions of hypophysis, thyroid gland, ovary and adrenal cortex, the evaluation of the urinary estrogen obtained from the females with regular menstrual cycles (7 cases) and with the menstrual irreguarity (11cases) by fraction technique, were carried out. The results are as follows: 1) In the females with regularity of menstrual cycles, the presence of the urinary estrogen was elicited throughout cycles, the highest levels of the same substance were maintained at the ovulation time and the lowest, being during the menstruation, avaerage levels were at 91 gamma/24 hrs. 2) In the cases with secondary amenorrhea the levels of estrogen were rather low in 3 cases out of 5, the remaining showing normal levels. In primary amenorrhea, all 2 cases revealed at lower contents. 3) In cases with irregularity, all 4 cases were observed to be maintained with normal levels. 4) The fractions of estradiol, estrone and estriol were found that the lowest levels were maintained by estradiol, the highest, by estriol.