뇨중 하수체성 G를 Crook법으로 정상월경주위를 가진 6예에서 월경기, 난포기, 배란기, 황체기, 4기로 나누어 GA. GB로 분리 측정했던 바 그 성적은 대략 아래와 같다. 1. 월경전주기를 통하여 GA. GB 양자 다 증명할 수 있으며 그 평균은 GA, 197γ/24hrs GB, 197γ/24hrs이다. 2. 배란기에서는 GA, GB 양자 다 peak를 정하며 GA, 360γ/24hrs GB, 142γ/24hrs이다. 3. 전주기를 통하여 GA/GB는 일정한 범위내에 있으며 그 범위는 2~3이다. 월경주기이상환자와 거세부인의 뇨중 G을 화학적인 방법으로 정량해서 정상량과 비교 검토하고 Estrogen투여전후의 변동에 대하여(특히 GA, GB의 소장) 관찰한 바 그 결과는 대략 다음과 같다. 1. 무월경환자의 G은 GA치, GB치, GA/GB가 정상과 다른 pattern을 보인다. 2. 월경불순, (희발성 무경) 환자에서는 GA가 높고 GB가 정상, 혹은 낮아서 GA/GB가 현저히 증가되어 있다. 3. 거세후 1~2개월에 있는 부인에서는 GB가 현저히 증가해 있다. 4. Estrogen 투여가 G에 미치는 영향은 GB에 더 현저하다. 5. Estrogen 10,000 I.U.를 연속투여하면 GA. GB 양자의 방출을 항진시키고 50,000 I.U. 이상 연속투여하면 양자의 방출을 억제한다. 6. Estrogen 20,000 I.U. 연속투여는 GB의 방출을 항진 혹은 억제시킨다.

In determination and evaluation of pitiutary gonadotropin, complicated biological methods for the assessment have been employed. However, recent advancement has clarified the physical and chemical nature of the substance. In cope with this advancement, a new quantitative analytical method, Crooke`s method, has been adapted enabling clinical use with the omission of complicacy in the procedure. Furthermore, this procedure has provided fractional analysis of gonado-tropin into GA and GB which correspond to FSH and LH respectively, and the knowledge of interrelationship and of balance between these fractions in various endocrinological environments. It has been well known that this procedure paved the way to establishment of diagnosis and therapeutic measures in case of functional disorders involving hypothalamus-pituitary-ovary system. By this chemical method, the levels of urinary gonadotropin in normal menstrual cycles and various irregular menstrual cycles were measured and the comparison between them were made. So called "feed back mechanism" upon hypothalamus-pituitary system, yet inconclusive, were reviewed from the view point of chage in urinary gonadotropin excretion following the administration of estrogen benzoate. The results are as follows. 1) The presence of gonadotropin in female urine obtained from the individuals with regularity of catamenial cycles and the highest level of gonadotropin were observed during the ovulation time. The average levels in urine were GA, 197γ/24hrs. and GB,80γ/24hrs. GA/GB was 2.5. 2) In comparison with the level of GA, GB, and GA/GB in normal cycles, the amount of GA and GB, also their ratio in patient with abnormal menstrual cycles difference. The levels of GB were especially deviated from normal ranges. 3) The adminaistration of estrogen benzoate were likely to be influential to the level of urinary gonadotropin in abnormally menstruating individuals, especially to GB. In case the estrogenic hormone 10,000 units (I.U.) given daily, increase of GA and GB were observed. On the contrary, administration of a single dose of more than 50,000 units of estrogen showed inhibitory effects. The amount of estrogenic hormone which acted as inhibitor, if given as single dose, would increase the amount of GA and GB in case of the same amount divided.