The suppression subtractive hybridization(SSH) was used to isolate stress-inducible genes from soybean. Using wounding-treated or low temperature-treated cDNA as a tester DNA, 2000 stress-inducible clones were obtained by SSH. Through dot blot hybridization of 1500 SSH-enriched cDNA clones, fifty wounding-induced clones and 100 low temperature-induced clones were isolated. Sequence analysis and database search showed that the isolated clones encode various kinds of stress-inducible genes, including heat shock-like protein homologue, cytokinin-repressed protein homologue, -amyrin synthase, lipoxygenase L-5, acyl CoA synthetase, DFR-like protein, and cold stress protein, etc. Northern blot hybridization showed that the transcription of W23(heat shock-like protein) and W51(cytokinin-repressed protein) were strongly increased within 6 hours after wounding. The expression of Lipoxygenase L-5 was restricted in systemic wounding of leaves. DFR-like protein was strongly influenced by circardian rhythm as well as drought stress rather than wounding. The highest expression of LT629(Cold stress protein) was revealed at 12 hours after low temperature treatment.