쇠고기 돼지고기 속에 포함된 nonylphenol, octylphenol, bits(2-ethylhexyl)phthalate의 동시 정량방법을 연구하였다. 이 방법에서는 밀폐된 배양시험관에서 이염화메탄을 이용하여 추출하고 용매를 이소헥산으로 바꾸고 aminopropyl 칼럼 (2g)을 이용하여 분리한 후 GCMS-SIM 방법으로 분석하였다. 다중점보정을 위한 nonylphenol(NP), octylphenol(OP), bis(2-ethylhexyl) phthalate(BEHP)의 회수율에서 NP, OP는 0.125-12.57 ㎍/g, BEHP는 0.0125-12.57 ㎍/g 범위에서 좋은 회수율을 보였다. 확립된 방법을 전주시와 전주시 근교 마트와 정육점에서 구입한 16점의 쇠고기 돼지고기 시료에 적용하였다. Nonylphenol은 4점의 시료에서 검출되었으며 bis(2-ethylhexyl)phthalate는 전 시료에서 검출되었고, 그 농도범위는 0.06-0.24 ㎍/g과 0.36-2.35 ㎍/g이었다. Octylphenol은 어떠한 시료에서도 검출되지 않았다. 이 방법은 매우 적은량의 생체 시료에도 쉽게 적용이 가능하였다.

A comprehensive analytical method of endocrine disruptors[i.e., nonylphenol(NP), octylphenol(OP), bis(2-ethylhexyl) phthalate(BEHP)] in meat or pork samples was developed. The method employed closed culture tube extraction with dichloromethane and solvent exchange to iso-hexane and SPE (2g) aminopropyl column, followed by determination on gas chromatography linked to mass spectrometry(GC/MS) operated in the single ion monitoring(SIM) mode. For the multipoint recovery of nonylphenol, octylphenol and bits(2-ethylhexyl) phthalate OP, NP were showen good recoveries in 0.125-1.25 ㎍/g range of concentration, and BEHP more good recoveries in 0.0125-12.5 ㎍/g wide range of concentration. The present method was applied to beef or pork samples of mart and butcher in Cheonju city and near Cheonju. The range of concentrations was respectively, 0.06-0.24 ㎍/g in nonylphenol(NP) and 0.36-2.35 ㎍/g in bis(2-ethylhexyl)phthalate(BEHP), but octylphenol(OP) was not dected in any samples. This method provides a powerful analytical tool to investigate a wide range of endocrine disruptors in biological samples of limited quantity.