The Amy locus (Amy, 2-80; 54A1-B1) of D. melanogaster is duplicated (Amy-p, Amy-d). The members of duplicated genes are ∼4 kb apart, consisting of 1482 by ORF without introns. They do not evolve independently but exhibit an evolutionary process called concerted evolution. To infer the concerted evolution, we analysed inversions of the Amy locus using PCR. In order to detect intergenic inversions, PCR primers were selected from highly divergent regions flanking Amy-p and Amy-d. The frequency of inversions in Suwon natural population was (7.018±0.001)×10^(-3). For genetic analysis, 6 homozygous lines of inversion mutants were made by mating with Cy/Pm. Homozygous inversion mutants were analysed by PAGE and Southern blotting. For detailed molecular analysis of Amy region in homozygous inversion mutants, we have constructed genomic DNA library from one mutant line and isolated two genomic clones for the Amy gene. The cloned genes were sequenced by dideoxy chain termination method. From the detection of the inversions in the Amy locus, we obtained some evidences of interchromosomal recombination or gene conversion.