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216.73.217.86
216.73.217.86
SCIE SCOPUS
Purification and Characterization of cAMP - Dependent Phosphoprotein 45 in Drosophila melanogaster
( Jeom Hee Lim , Kyoung Sang Cho , Chung Choo Lee )
Genes & Genomics 19권 4호 338-338(1pages)
UCI I410-ECN-0102-2009-470-007057649
이 자료는 4페이지 이하의 자료입니다.

Tissue-specific cAMP-dependent phosphoproteins (pp45, pp40, and pp20) were detected in accessory gland. In the present study, we characterized and purified pp45. The purification procedure consisted of 3 steps including heat treatment, DEAE cellulose column chromatography and high performance liquid chromatography (HPLC). The purified protein could be phosphorylated in vitro by the catalytic subunit of cAMP-dependent protein kinase and derived from the lumen of the accessory gland. The partial sequences of this protein were lso-Lys-Asn-Val-Ala-Lys-Ala-Glu-Arg-Asn-Met-His-Asn-Met-Leu-Arg and showed 6 amino acids sequence homology with Mst57Dc. Currently, we are performing cDNA library screening and making the antiserum of pp45.

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