The chromosomal DNA fragments of Corynebacterium glutamicum ATCC 13058 containing genes involved in histidine biosynthesis were cloned in pBluescript SK(+). To investigate the structural organization and the regulation of these genes, recombinant clones complementing E. coli hisA, hisB, hisC, hisI and hisF mutants were isolated from genomic library. A recombinant plasmid containing a 5.6kb fragment of genomic DNA was able to complement E. coli hiA, hisB and hisF. But pCGH 13, carrying insert of 1.7kb complemented only E. coli hisC. The genetic data indicate that these genes are clustered in a small region f the chromosome. Nucleotide sequence analysis of the 1.7kb fragment of genomic DNA revealed one putative open reading frame which showed significant homology with the hisC gene of E. coli K-12 and Lacctococcus lactis.