During transplant operation, we need specific antibodies against HLA antigens for the HLA typing between donor and recipient. On the production of the anti-HLA monoclonal antibody in the human system, up to this point available partner cell was not developed. We must develop more available fusion partner cell, therefore we fused SKO-007, human myeloma, and HeLa cell, human cervix carcinoma. We made more effective clones as human fusion partner cells. As a result, their doubling time is shorter than SKO-007 and they are 6-thioguanine resistant. The most available clone, HS-7, of them has the shortest doubling time, 35 hours, and its chromosome number is 70. It has been using for the production of the anti-HLA monoclonal antibody secreting hybridoma. It can be used as fusion partner cell for production of several monoclonal antibodies.