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18.97.9.171
18.97.9.171
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SCIE SCOPUS
Molecular Cloning of Rat Steroidogenic Acute Regeulatory Protein and Its Constitutive Expression in Rat Leydig Tumor Cells , R2C
( Hye Kyung Lee , Hyuk Bang Kwon , Jae Mog Soh )
UCI I410-ECN-0102-2009-470-007057189
This article is 4 pages or less.

The expression of steroidogenic acute regulatory (StAR) protein is up-regulated in steroidogenic cells in acute response to trophic hormones and the protein is responsible for the mobilization of cholesterol from mitochondrial outer membrane to inner membrane where cholesterol side chain cleavage enzyme reside. Rat complementary DNA corresponding the StAR protein was cloned and the complete nucleotide sequence was determined. The cDNA is consisted of 1560 nucleotides and has the longest open reading frame of 320 amino acids. The deduced amino acids sequence show high degree of homology to mouse, bovine, and human StAR protein in amino acid level. Rat tissue Northern blot showed that it is only expressed in testis, ovary, and adrenal gland. Rat Leydig tumor cells, R2C can produce testosterone without trophic hormone stimulation or cAMP analog treatment. In order to correlate the constitutive production of the hormone with the the level of StAR mRNA, we analyzed the expression of the StAR mRNA in rat R2C cells. The Northern blot showed that R2C cells expressed the StAR mRNA without stimulation and treatment of cAMP did not increase the level of StAR mRNA, which indicates that constitutive steroidogenesis are caused by high basal level expression of StAR mRNA and StAR expression in R2C cells is not dependent on cAMP level.

[자료제공 : 네이버학술정보]
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