We have previously reported that the orphan nuclear hormone receptor SHP act as a heterodimeric partner of several nuclear hormone receptors and it inhibits the activity of these receptors. In order to study the gene regulation mechanism, we have cloned the genomic DNA of SHP from mouse and human genomic library. Exon-intron structure of mouse and human SHP genomic DNA are determined. Both genomic structure contain two exons and one intron. 2.3 kb of mouse and 1.3 kb of human SHP gene. Several potential transcription factor binding sites such as SP-1, AP-1, GATA, ROR, and NF_(κ)B exist in mouse SHP promoter region. SHP gene is localized in human chromosome number 1 by somatic cell hybrid panel and fluoresence in situ hybridization (FISH) analysis.