닫기
18.97.9.173
18.97.9.173
close menu
}
SCIE SCOPUS
Genetic Polymorphism of HLA - DPB1 Gene Using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism in Korean
( Sung Hoi Hong , Kyung Ok Lee )
UCI I410-ECN-0102-2009-470-007057149
This article is 4 pages or less.

The HLA-DP genes are situated at the centromeric end of the major histocompatibility complex (MHC) in humans. The DP α-β heterodimers, like other HLA class II antigens (DR and DQ) bind to foreign or self antigenic peptides and specify heterodimeric glycoproteins involved in the regulation of the immune response. In this study, an comprehensive HLA-DPB1 genotyping method using PCR-RFLP were developed. Second exon of DPB1 was selectively amplified from genomic DNAs of 18 homozygous B-cell lines and unrelated Koreans by PCR. Amplified DNA were digested with 7 allele specific restriction enzymes - EcoNI, RsaI, FokI, DdeI, Sdul, BbvI and BssHI. The results of 18 control cells correlated well with the data which were previously reported. In a total of 28 different DPB1 alleles, the most frequent allele was DPB1*0501 among Koreans. This study shows that this method is relatively simple, fast and practical tool for the HLA-DPB1 genotyping. Moreover the result of HLA-DPB1 genetic polymorphism might be useful for the database study before using for disease association, individual identification and transplantation immunity.

[자료제공 : 네이버학술정보]
×