Using PCR amplification, ABO blood genotype and the gender of unknown specimen could be determined. The genomic fragments coding ABO related enzymes, 3-N-acetylgalactosaminyl transferase and 3-galactosyl transferase, were amplified and subsequently digested with corresponding restriction enzymes. Comparing restriction pattern of each fragment, ABO blood genotype was determined. The intron part of amelogenin gene located on sex chromosome was also amplified in order to verify the gender of specimen. The male showed heterozygote band pattern while female showed homozygote due to the length variation of intron in each X and Y chromosome. These results will provides useful tools for the forensic identification and genetic analyses.