The short tandem repeats (STR) region are informative markers for the genetic characterization of individuals and personal identification in forensic science. In this study, the allelic distribution of four tetranucleotide repeat STR loci; vWF, TH01, TPOX, CSF1PO have been determined by amplified fragment length polymorphism(Amp-FLP) from unrelated Korean individuals. The results showed that our population data satisfied HardyWeinberg equilibrium expectations. Some statistical value which determine the usefulness of each locus for forensic identification were also determined. The results from this research are in use nowadays in forensic identification of our laboratory.