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18.97.9.175
18.97.9.175
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SCIE SCOPUS
The Phenotype of a Temperature - Sensitive Mutant of Saccharomyces cerevisiae ARS - Binding Fator 1 Gene is Suppressed by TRP1 Gene at High - Copy Number
( In Seop So , Ji Young Kim )
UCI I410-ECN-0102-2009-470-007056940
This article is 4 pages or less.

ABFI is a DNA binding protein that recognizes the motif, RTCRYN_5ACG, at many sites in the yeast genome including autonomously replicating sequences (ARSs), mating type silencers and promoter elements. YEP24-SAB1 plasmid containing a suppressor gene for temperature-sensitive lethal mutant in the ABFI gene was isolated by their ability to permit growth of abf1-5 ts-mutant at non-permissive temperature. Hybridization to Chromoblot and to prime clone grid filters revealed that SAB1 gene was located on chromosome IV. To localized the suppressor activities on the cloned plasmids, some deletions were made and analyzed. The suppressor activity was narrowed down to 1 kbp yeast genomic DNA. The nucleotide sequence of the 1 kbp DNA was determined and turned out to contain TRPI gene encoding phosphoribosylantranilate isomerase.

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