The use of restriction fragment length polymorphisms (RFLPs) has been proposed for the construction of a human genetic linkage map and also become a powerful tool in human population genetics. Human Y-chromosome which is haploid and paternally inherited is valuable for investigating male-mediated gene flow and for complement maternally based studies of mtDNA. We have studied 166 samples from 4 kinds of family origin names such as Kyungju Kim, Kimhae Kim, Chunju Lee and Milyang Park based on the 49aTaq 1 polymorphisms, and a total of 25 haplotypes were observed. The B, F, and I bands is observed in all haplotypes and haplotype showing A3, B, D2, D3, F, and I bands is common haplotype except for Chunju Lee. The result obtained are summarized as follows.