The Amy locus (Amy, 2-80; 54A1-B1) of D. melanogaster is duplicated (Amy-p, Amy-d). The members of duplicated genes are ∼4 kb apart, each consists of 1482 by ORF without introns and do not evolve independently but exhibit an evolutionary process called concerted evolution. To infer the concerted evolution, we analysed inversions of a Amy locus using PCR. In order to detect intergenic inversions, PCR primers were selected from highly divergent region in flanking regions of Amy-p and Amy-d. The frequence of inversions in Canton-S was 2.77 × 10^(-3). For genetic analysis, five inversion mutants were made homozygous for chromosome II using a balancer chromosome Cy and routine crosses. Homozygous inversion mutants were analysed by PAGE and Southern blotting. From the detection of the inversions in Amy locus, we obtained evidence of interchromosomal recombination or gene conversion.