A genetic population study for the tetrameric STR, the HUMCYAR04 in the Korean population was carried out. Typing for the HUMCYAR04 of 236 unrelated Koreans was done by PCR and PAGE-silverstaining. Eight alleles were observed and their frequencies were in the range of 0.0042 - 0.3491. Allele 5, 6, and 10 were higher frequencies than other alleles. No significant deviations from Hardy-Weinberg equilibrium could be observed in the genotype distribution. The observed heterozygosity was 0.7426 and the PIC value was 0.7268. The power of discrimination (PD) was 0.8906 and the chance of exclusion (CE) was 0.4809. There is no significant difference in the allele frequencies at the HUMCYAR04 locus between Koreans and Asians. However, the Koreans did slightly differ for the allele frequencies when compared with Mexican-Americans, Blacks, and Caucasians.