The polymorphisms of genetic markers based on the variable number of repeat units are displaying considerable polymorphisms and providing the complementary information. In the present study, twenty-one short tandem repeat (STR) markers based on (dC-dA)n (dG-dT)n dinucleotide repeat sequences were analyzed for their polymorphisms in the Korean population using fluoroscently labeled primers and automated sequences with Genescanner software. The potential usefulness of these polymorphisms for the determination of individual identification was calculated from the allele frequencies of twenty-one STR markers. The most common genotype frequency combined of twenty-one markers was 6.09×10^(-11) and the least common genotype of a random match (PM) combined of the twenty-one markers was very low with 7.48×10^(-24). The discrimination power was high showing above 0.8 with the exception of D20S115 (0.737) and was the highest showing 0.954 in D12S85 and D20S117. The STR genotyping by the fluorescence-based system significantly facilitates large-scale databasing for the individual identification. Therefore we can obtain the high discrimination power for the individual identification within a short time frame.