We have analysed the allelic frequency distribution at the short tandem repeat (STR) to the CD4, TH01, vWA (vWA31A) loci in Human DNA by PCR/FLP techniques. In a sample of 200 unrelated Koreans, the CD4 locus demonstrated a heterozygosity of 43 with 5 alleles and 7 genotypes. The allelic diversity value and the PD of CD4 were found to be 0.44 and 0.61 respectively. The TH01 locus demonstrated a heterozygosity of 70.3% with 5 alleles and 14 genotypes. The allelic diversity value and the PD of TH01 were found to be 0.71 and 0.80 respectively. The vWA locus demonstrated a heterozygosity of 79.9% with 7 alleles and 23 genotypes. The allelic diversity value and the PD of vWA were found to be 799 and 0.97 respectively. Comparison of the result an allele frequencies with other published databases showed that their distributions were not similar. Observed heterozygosity was similar to that reported in other population studies but significantly higher than expected. These population data will allow the use of the 3 STR marker in paternity determination and the analysis of individual identity in forensic samples