We had previously isolated cDNA clone, PVR3 encoding root-specific ns-LTP (non specific lipid transfer protein) like protein from Bean (Phaseolus uulgaris L.). In this study we isolated genomic DNA corresponding to PVR3 cDNA by IPCR (inverse PCR) method. On the basis of Sourthern blot analysis, the genomic DNA was digested with HindIII, self-ligated, digested with BamHI and used as template for IPCR. Primers for IPCR were designed towards sequence at the 5`-end of coding region. IPCR product contains a coding region identical with that of PVR3 cDNA and no intron is revealed. A putative TATA box is located at position -69 and several palindromes and tandem repeat sequences are present. There is also one TGACG motif which are known as the root-specific sequence of CaMV 35S. Further analysis of PVR3 promoter is in progress by construction the transgenic plants which have 5`-flanking region combined with the GUS reporter gene.