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SCIE SCOPUS
Mutagenesis and Analysis of Fusogenic Activity of Autographa californica Nuclear Polyhedrosis Virus ( AcNPV ) gp64 Envelope Fusion Protein
김희진 , 양재명
UCI I410-ECN-0102-2009-470-007056319
This article is 4 pages or less.

The baculovirus gp64 glycoprotein is a major component of the envelope of budded virus (BV) and has been shown that it plays an essential role in the infection process, especially virus-cell membrane fusion. We have cloned AcNPV gp64 gene and expressed transiently in transfected insect cells. The cells expressing gp64 protein were examined for membrane fusion activity by using a syncytium formation assay under various conditions. The followings are optimal conditions required for inducing membrane fusion; 1) a pH 4.6 or lower; 2) 15 min exposure time under acidic pH; 3) at least 1 ㎍ of gp64 cloned plasmid DNA; 4) expose the cells to acidic pH at 72 hr post-transfection. In order to investigate the role of hydrophobicity for the membrane fusion, the two leucine residues (amino acid position at 229, 230) within hydrophobic region I were mutated to alanine by PCR and the membrane fusion activity of the mutant was analysed.

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