2 a number of neurological mutations of the fruit fly, Drosophila melanogaster induced by EMS have been extensively characterized. Among them, X chromosome-linked temperature-sensitive mutant shibire (shi) displays paralytic behavior at restrictive temperature. Another class of X-linked recessive mutants, bang senseless (bss), easily shocked(eas), and technical knock-out(tko) exhibit paralysis on mechanical vibration. The behavioral phenotypes of bang-sensitive and temperature-sensitive mutant flies to their corresponding stimuli are very similar to each other. However, substantial differences were observed in the behavioral repertoire during paralysis and recovery and recovery time. The changes of bang-sensitivity in both sexes and at various ages of bss, eas and tko flies were quantitatively analyzed. Furthermore, the functional relationship in the nervous system between bang-sensitive and temperature-sensitive mutations has been examined in the shi·tsl·bss double mutant flies. shi·tsl·bss flies showed an increased bang-sensitivity when compared with single mutant flies of shi·tsl or bss. This indicates a synergistic effect of the double mutant on bang-sensitivity. Our results suggest that the temperature-sensitive mutant shi is also associated with bang-sensitivity and the gene product of shi and bss may affect different cellular processes in the nervous system.