The discovery of biochemical polymorphisms has contributed greatly to a better understanding of the genetic characteristics of human populations. The distribution of these characteristics has been analysed in large groups of populations in many ethnic groups. In Korean populations, several protein polymorphisms have been reported. These proteins were group specific component in plasma, transferrin C subtypes and haptoglobin phenotypes in serum. These studies on protein polymorphism were conducted mainly using blood. So, variation and gene frequencies of parotid middle-band protein were examined to study salivary protein polymorphism of Korean population. There were two phenotypes; Pm(+) and Pm(-), and their frequencies were 0.384(Pm+) and 0.616(Pm-).