Allozymic variations at eight polymorphic loci in five species belonging to takahashii species subgroup were analysed through horizontal starch gel electrophoresis. Phylogenetic relationships based on NEI's as well as NAIR's indices revealed two main lineages. In one of the lineage, D. paralutea and D. prostipennis constitute one group while other main lineage includes two closely related species i.e. D. takahashii and D. lutescens and one distantly related species i.e. D. nepalensis. Thus, observed phylogenetic relationships based on allozymic data concur with the interrelation based on interspecific hybridization tests. The extent of observed allozymic divergence among five species of takahashii subgroup seem to be correlated with their allopatric and endemic geographical distribution pattern throughout the oriental region.