A human stromelysine II gene derived cDNA was used as a probe for in situ hybridization to the chromosome of the three lemur species, Eulemur fulvus myottensis, Eulemur macaco macaco and Eulemur coronatus. This sequence, a member of collagenase gene famaly locating in cluster at human chromosome 11q23-25, was found on homologous bands of three morphologically similar chromosomes segments, Eulemur fulvus, Eulemur macaco and Eulemur coronatus, confirming that different Robertsonian translocation occurred during the evolution of these three species. The Stromelysine II gene is located at q21-23 of the ancestor chromosome 5 of lemurien.