Leaf mesophyll protoplasts isolated from amphidiploid Allium wakegi were fused with protoplasts derived from young leaf sheath bases of A. fistulosum. The somatic hybrid cell was cultured in KM8P medium supplemented with 0.4M glucose, 0.1M mannitol, 0.4mg/l 2, 4-D, 2mg/l NAA and 1mg/l BAP for 5 days. Then the medium was diluted by adding fresh KM8P medium with 1g/l glutamine(substituted for ammonium nitrate and potassium nitrate), 0.4M glucose, 1mg/l NAA and 1mg/l BAP. After three days the medium was diluted with KM8P medium with 1g/l glutamine, 0.3M glucose, 0.1mg/l NAA and 1mg/l BAP. When MS medium with 30g/l sucrose, 0.1mg/l NAA, and 1mg/l BAP was added into the petri dishes in three days intervals, and callus was obtained.