Vectors with Clostridium thermosulfurogenes α-amylase gene as a foreign gene and E. coli hph gene as a selectable marker gene which represented hygromycin Resistance(HMBR) were constructed. AlcA promoter and trpC terminator of Aspergillus nidulans were used on these vectors. Sensitivity of Aspergillus nidulans KCTC6034 to HmB was checked. A. nidulans did not grow at the plates with 600μg/ml of HmB. A. nidulans was transformed with these vectors. Twenty two transformants were obtained and screened by polymerase chain reaction with genomic DNA to confirm the existence of the foreign DNA. Most of transformants had hph gene sequence and at least 26% of transformants had α-amylase gene sequence.