The intergenic sequence between rbcL and atpB genes of chloroplast DNA were sequenced from five individual trees, one individual each of Monterey pine (Pinus radiata) , knobcone pine (Pinus attenuata), and three geographic groups of bishop pine( Pinus muricata). No variation was observed among the 667 bp intergenic sequences of five individuals which had been directly sequenced with the PCR(polymerase chain reaction)-amplified DNA. Alignment of the upstream sequences (251 bp) of the rbcL gene with those of Douglas-fir showed bout 88.4% similarity.