Organogenetic ability in callus culture and the karyotypes in regenerated plants derived from the callus of Allium sativum L. were analyzed. Organogenetic ability in callus culture depended on the duration of culture on the medium with 2, 4-D and organogenesis-inducing medium (IAA+Kinetin). 347 regenerants out of total 467 were analyzed for chromosome numbers. They consisted of diploid, tetraploid, aneuploid and mixoploid as a frequency of 287 (0.83), 51 (0.15), 5 (0.01) and 4 (0.01) , respectively. Karyotypic analyses were carried out in 169 plantlets whose chromosome numbers checked. Normal diploid, abnormal diploid, normal tetraploid, abnormal tetraploid, aneuploid and mixoploid were found to be 113 (0.67), 29 (0.17), 15 (0.09), 4 (0.02), 4 (0.02) and 4 (0.02), respectively. Chromosomal variations in number and structure were observed in abnormal diploid and tetraploid. Chromosome additions were mainly found in a, b, c, d, e and h chromosome but deletions in f and g chromosome. Elongated a chromosome, different from of h chromosome and very small chromosome were also observed in regenerated abnormal diploid. Deleted short arm in d chromosome were found in abnormal tetraploid. Short arm deletions of satellite chromosome were observed in abnormal diploid and tetraploid regenerants.