In a series of genetic studies on the Korean population, the genetic polymorphisms and allele frequencies of phosphoglycolate phosphatase (PGP) in the red blood cell hemoglobin obtained from 1,077 individuals were analysed by the 6% horizontal polyacrylamide gel electrophoresis. Three electrophoretic phenotypes, PGP 1-1, PGP 2-1 and PGP 3-1, were observed as products of the different combinations of three alleles, PGP*1, PGP*2 and PGP*3 at an autosomal locus, PGP. Based on the investigation of 1,077 unrelated individuals the following gene frequencies were calculated. The allele frequencies of PGP*1, PGP*2 and PGP*3 were 0.9921, 0.0051 and 0.0028, respectively. It was reported that the PGP*1 allele was the dominant in all of the racial populations studied with the range of 0.8233 and 0.9921, and the frequency of PGP*3 allele seems to be the lowest in the Korean population in the world with exception of the Caliari, Rome population (0.0000).