The karyotypes in callus cells and regenerated plants derived from the callus of Allium sativum L. were analyzed. The leaflet discs of A. sativum proliferated into sufficient callus for subculturing after 120 days of initial culture. Karyotypical analysis was done every 30-day interval. Among the various ploid levels observed in the callus, diploid cells were most frequent and other ploid cells appeared in more or less low frequencies. The regenerated plants were diploid, tetraploid or mixoploid. The mixoploid showed a tendency to be stabilized as diploid or tetraploid as they grow.