Crown gall tumors of Phaseoulus vulgaris cv. Palgong induced by Agrobacterium tumefaciens C58 were cultured in vitro and analyzed for their soluble proteins by two-dimensional polyacrylamide gel electrophoresis. There were some differences in soluble proteins between a hypocotyl-originated normal cell line and tumor-originated cell lines. In the tumor cell line grown on the hormone-free medium, three protein spots were newly induced, two disappeared, five reduced, and eleven increased in comparison with the normal cell line. The effect of exogenous picloram on soluble proteins was also investigated in tumor cell line. In tumor cell line treated with 2.5μM picloram or 5μM kinetin, protein patterns appeared similar to those of the normal cell line.