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18.97.9.174
18.97.9.174
SCIE SCOPUS
Transformation of HPRT - Deficient Mouse Cell with Human HPRT cDNA by DNA - Mediated Transfection
( Chang Bo Ko , Yung Jin Kim , Sang Gi Park )
UCI I410-ECN-0102-2009-470-007054653
This article is 4 pages or less.

Transfer of the human hypoxanthine phosphoribosyltransferase (HPRT) gene into the murine myeloma cells in vitro was accomplished with retrovirus-derived vectors. To construct expression plasmids for the human HPRT gene, human HPRT cDNA was ligated into murine retroviral vector, pRSVneo vector conferring resistance to neomycin-kanamycin (Tn5) antibiotics and viral long terminal repeats. Vectors of subcloned and modified from it were introduced into cultured HPRT deficient murine myeloma cell (SP2/0) by calcium phosphate-mediated DNA transfection. The transformed cells were selected against G418 or hypoxanthine/aminopterin/thymidine(HAT) medium and were used for HPRT enzyme activity. The HPRT activity in the lysates of the transformed cells were increased more ten-fold than that of HPRT-deficient SP2/0 cells. we now investigate whether these transformed cells contain authentic human HPRT or not.

[자료제공 : 네이버학술정보]
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