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216.73.217.86
216.73.217.86
SCIE SCOPUS
Actin Gene in Red Pepper ( Capsicum annuum )
( J . K . Park , C . B . Hong , W . S . Shin )
Genes & Genomics 12권 4호 385-385(1pages)
UCI I410-ECN-0102-2009-470-007054188
이 자료는 4페이지 이하의 자료입니다.

An actin gene from red pepper was cloned. Polyadenylated RNA purified from red pepper seedling was assayed for the actin mRNA by Northern hybridization with the Dictyosterium discoideum actin cDNA clone as a probe. Double-stranded cDNA synthesized from the polyadenylated RNA was cloned in EcoRV site of pBR322, and the cDNA bank was screened with the actin cDNA probe of D. discoideum. The putative clone was characterized by Northern hybridizations. The clone was designated as pRPAcI. We determined the nucleotide sequence of pRPAcI for the carboxy terminal half of the protein. The deduced amino acid sequence has high homology with other plant actins.

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