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SCIE SCOPUS
억제 유전자를 포함치 않은 효모의 절제회복 관여 RAD4 유전자의 분리
The Isolated RAD4 Gene Required for Nucleotide Excision Repair in Saccharomyces Cerevisiae Does Not Contain a Suppressor Gene
최인순 , 김재범 , 전성호 , 박상대 ( In Soon Choi , Jae Bum Kim , Sung Ho Jeon , Sang Dai Park )
Genes & Genomics 12권 2호 147-155(9pages)
UCI I410-ECN-0102-2009-470-007038504

The RAD4 gene of Saccharomyces cerevisiae is absolutely required for the incision step of UV-induced nucleotide excision repair. The previsously cloned RAD4 gene by phenotypic complementation of UV-sensitive rad4-4 mutants with a yeast genomic library (pPC1) appeared to localize in the right arm of chromosome V as determined by rapid chromosome mapping This cloned RAD4 gene did not contain a suppressor gene as determined by suppression test of various auxotrophic mutants containing nonsense mutations. In addition, all different plasmids harboring the cloned yeast DNA inserts conferred UV resistance to rad 4-4 mutants to a similar level regardless of their copy number. These results indicated that the pPC1 is a truly functional RAD4 gene and its complementing activity must be resulted from the yeast RAD4 gene.

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