Giemsa C-banded karyotypes on the 200 regenerated Allium wakegi were analyzed. About 92.5% of the regenerated plants were amphihaploid plants, and the C-banding patterns of these plants were almost same as that of the common species. But four types of variants could be classified on the basis of morphological characteristics and C-banding patterns. Amphidiploid variants which is composed of 5% of the total regenerated plants showed doubled C-banding karyotypes of amphihaploid one. Four types of the amphidiploid variants exhibited a chromosomal rearrangement identified by C-band distribution. Hypoamphidiploid variants of 2n=28 were also obtained, but they showed low fitness to the environmental factors. Amphidiploid plants showed better viability, growth and larger bulb than the common cultivar plants.