The replication sequences of Autographa californica nuclear polyhedrosis virus (AcNPV) ts-B1074 were comparatively visualized at time intervals from 18 to 48 hours postinfection at 25℃ and 32.5℃. the late stages of the multiplication sequence were quite different in the amounts of virogenic stroma formation and nucleocapsid formation, polyhedra occlusion body formation, and occlusion of enveloped virions at 25℃ and 32.5℃. The amounts of virogenic stroma formation decreased and the amounts of NOVs remained more in the nucleus at 32.5℃. The polyhedra appeared early by 18 h postinfection at 32.5℃, but later by 24 h postinfection. The formations of the polyhedra and the occlusion of the enveloped virions onto the polyhedra were more defective and temperature-sensitive at 32.5℃. Bundling of nucleocapsids and cytopathic effects of virions were not different at either temperature. But the amounts of extracellular NOVs were present much more at 25℃. The mutant had defects in the late genes.