Replication sequences of Autographa californica Nuclear Polyhedrosis Virus ts-B1074 at 25℃ and 32.5℃ were comparatively visualized at time intervals from 0 to 48 h postinfection. The nucleocapsids adsorbed onto the host cell surface within 30 min, penetrated into the cytoplasm within 60 min and passed through the cytoplasm to the nuclear membrane within 2 h postinfection. Eclipse period was occurred from 4 to 10 h postinfection. At 10 h virogenic stroma were formed. At 13 h postinfection, progeny nucleocapsides were appeared and budded. At 18 h prepolyhedral proteins were appeared. At 30 h the normal polyhedra were matured and occluded by enveloped nucleocapsids, but the polyhedra of the ts mutant were abnormal at 32.5℃. Therefore the enveloped nucleocapsids were not occluded onto them.