Interleukin 2 (IL-2) is a glycoprotein of 15500 daltons molecular weight produced by T-lymphocytes, which stimulates cells from hepatopoietic lineages which express specific IL-2 receptors. Monoclonal antibody against IL-2 peptide was generated 104 wells among 218 which were survived in HAT media produced anti IL-2 antibody. All 11 clones which were recloned from 104 positive wells have been identified to produce IgG₁ type monoclonal antibody which has kappa light chain after ELISA subtyping analysis. After purification of IgG₁ monoclonal antibody, heavy and light chain of immunoglobulin has been identified whose molecular weight are 51 KD, 25 KD, respectively. These IL-2 antibodies were confirmed using immunoblotting techniques. IgG₁ type Il-2 monoclonal antibody were bound to IL-2 antigen whose molecular weight is 15.5 KD.