Temperature affects the rate of development of an organism. to investigate the effects of the extreme temperature on the developmental profiles in Drosophila melanogaster vestigial mutant, the early first-instar larvae were incubated at temperature extreme (31℃) for 2 days and transferred thereafter to moderate temperature (24℃), and the enzyme activities and the changes in the eletrophoretic patterns of glycerol-3- phosphate dehydrogenase and lactate dehydrogenase were studied throughout the developmental stages. The thermophene that has relatively normal wing was induced by temperature treatment from vestigial phenotype, but the life span of adult thermophene flies was shortened. The enzyme activities of glycerol-3-phosphate dehydrogenase and lactate dehydrogenase during development were markedly influenced by temperature treatment in vestigial mutant comparing with the normal wild type flies; however, the electrophoretic patterns of both enzymes were not changed.