A karyological analysis was carried out on different Korean species of the family Gobiidae (10 genera, 3 subfamies). The preparations were made according to the flame drying method, and the karyotypes were analyzed by conventional Giemsa staining. Diploid chromosome number ranged between 40 and 50 (Acanthogobius hasta, 44; A. lactipes, 40; A. flavimanus, 44; Acentrogobius pflaumi, 50; Rhinogobius brunneus, 44; Chasmichthys dolichognatuhs, 44; C. gulosus, 44; Chaenogobius annularis, 44;C. castaneus 42; C. mororanus, 42; Mugilogobius abei, 46; Favonigobius gymnauchen, 48; Odontobutis platycephala, 44; Tridentiger obscurus, 44; T.sp. , 44), and arm number was in the range of 40-96. Some detected chromosomal characteristics permitted establishing some karyotypic relationships among the different species investigated.