Total tRNAs of A. nidulans were extracted by hot phenol extraction and purified with DEAE cellulose chromatography. The specific tRNAs were separated on the two-dimensional polyacrylamide gel. tRNA from single spot was eluted and labeled with r-^(32) P-ATP. After partial digestion with RNases (T₁, U₂, Phy L, B. cereus, CL 3), each sample was loaded on the sequencing gel. From the sequencing data, the testing tRNA was identified as tRNA^(Arg)_(ACG). tRNA^(Arg)_(ACG) shows considerably more homology with yeast (60% homology) than with prokaryotic (E. coli; 30% homology). The invariable sequences were observed. Further sequence analysis is going on by TLC method for the identification of modified bases.