Changes in amylase, cellulase, and proteinase in the cotyledons and embryo axes of soybean(Glycine max cv. Eunhakong) seeds, were determined over a 6-day germination period. Day-0 samples of seeds were soaked in the strong oxidant of ozone treated water(0.3ppm) for 4 hour and cultured in ozone water(six times a day) of 25℃ in a germinator. In the non-treatment of ozone water, amylase and cellulase activity of embryo axes decreased more rapidly throughout the germination period while those of cotyledons increased steadily during the first 4 days and declined thereafter. And total amylase and cellulase activity of the sprouting seeds decreased steadily during the first 4 days and decreased rapidly after the fourth day. Proteinase activity of embryo ayes and cotyledons increased slightly during the germination period, but those of embryo axes decreased steadily after the fourth day. In the treatment of ozone waters, amylase of cotyledons was high activity during the first 2 days and was low activity thereafter in comparison to non-treatment while that of embryo axes was low activity only in the first days and was similar activity thereafter. Cellulase of embryo axes and cotyledons was low activity during the 2∼4 days while that of embryo axes was high activity in the first days and the 6 days in comparison to non-treatment. Proteinase of cotyledons was high activity throughout the germination period while that of embryo axes was low activity during the first 2 days and was high activity thereafter in comparison to non-treatment.